Fibroblast Activation Protein Antibody
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Datasheet

Fibroblast Activation Protein Antibody

Rabbit Polyclonal

600-401-A73S 600-401-A73
25 µL 100 µg
ELISA, WB
Mouse
Rabbit
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$99.00 /Per Item
$399.00 /Per Item
25 µL $99.00
100 µg $399.00
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Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP)  is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules.  FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4.  Multiple isoforms of FAP are reported as alternative splicing products from a common gene.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 88 kDa in size corresponding to FAP by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  This antibody is known to react with mouse FAP protein.  A BLAST analysis was used to suggest partial cross-reactivity with FAP from human and rat sources based on ~88% homology with the immunizing sequence.    Reactivity with FAP from other sources has not been determined.

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Fibroblast Activation Protein (FAP) (RABBIT) Antibody - 600-401-A73
rabbit anti-Fap antibody, Fibroblast activation protein alpha, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Integral membrane serine protease antibody, FAPA antibody, Gelatine degradation protease FAP, Surface-expressed protease, SIMP, Seprase, APCE
Fap
ELISA, WB
Mouse
Mouse
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse FAP protein.
Liquid (sterile filtered)
Dry Ice
Expiration date is one (1) year from date of opening.
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.