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Datasheet

Fat Free Antibody

Rabbit Polyclonal

600-401-BD7
100 µg
WB, ELISA
Human, Mouse, Rat
Rabbit
$475.00 /Per Item
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Background

Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN). Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

Product Details

Anti-Fat Free (RABBIT) Antibody - 600-401-BD7
Fat Free homolog Antibody, FFR, ANG2, ANG3, C11orf2, C11orf3, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein, Protein fat-free homolog, VPS51
Rabbit
Polyclonal
IgG

Target Details

VPS51  - View All VPS51 Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Fat Free antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid synthetic peptide near the C-terminus of human Fat Free.
Anti-Fat Free Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.

Application Details

ELISA, WB
Anti-Fat Free Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 86 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.