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ERK-MAPK phospho T202/phospho Y204 Antibody

Rabbit Polyclonal

600-401-C76
100 µL
Liquid
WB, IHC
Human, Mouse, Rat
Rabbit
$475.00 /Per Item
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Product Details

Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody - 600-401-C76
Mitogen-activated protein kinase 1, MAP kinase 1, MAPK1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42
Rabbit
Polyclonal
IgG

Target Details

MAPK1/2  - View All MAPK1/2 Products
Human, Mouse, Rat
Phosphorylation
Conjugated Peptide
Anti-ERK/MAPK Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.
ERK/MAPK pT202/pY204 antibody is directed against human ERK/MAPK. ERK/MAPK pT202/pY204 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase. Reactivity from the following species based on 100% sequence homology: bovine, canine, chicken, mouse, non-human primates, Xenopus and zebra fish.
P63086 - UniProtKB
NP_446294.1 - NCBI Protein

Application Details

IHC, WB
Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42-44 kDa in size corresponding to ERK/MAPK phosphorylated at Thr202 and Tyr204.

Formulation

Titrated value sufficient to run approximately 10 mini blots.
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-ERK/MAPK pT202/pY204 Antibody detects ERK/MAPK which is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204. Anti-ERK/MAPK pT202/pY204 Antibody is ideal for investigators involved in Cell Signaling and Cancer Research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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