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DYKDDDDK Tag (Anti-FLAG®) Antibody Dylight™ 549 Conjugated
Mouse Monoclonal 29E4.G7 IgG2a kappa
200-342-383
100 µg
Lyophilized
WB, ELISA
FLAG-Tag
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Antibody for the detection of FLAG® conjugated proteins (MOUSE) Monoclonal Antibody DyLight™ 549 Conjugated - 200-342-383
mouse anti-FLAG™ tag DyLight™549 conjugation, Enterokinase Cleavage Site (ECS), DyLight™549 conjugated mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
Mouse
DyLight™ 549
Monoclonal
IgG2a
Target Details
FLAG-Tag
Conjugated Peptide
This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
This antibody is directed against the FLAG™ epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the FLAG™ epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Application Details
ELISA, WB
Anti-FLAG DyLight™549 has been tested by ELISA and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent western blotting fluorescent polarization and FRET. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This antibody is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.