Dab1 Antibody
IHC - Dab1 Antibody
WB - Dab1 Antibody
WB - Dab1 Antibody
Western Blot of Anti-Dab1 (400-555)
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Datasheet

Dab1 Antibody

Rabbit Polyclonal

8 References
100-401-225S 100-401-225
25 µL 100 µL
WB, IHC, IF, Biochemical Assay, Multiplex
Mouse
Rabbit
$125.00 /Per Item
$475.00 /Per Item
25 µL $125.00
100 µL $475.00
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Background

Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'.  Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes.  Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development.  Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain.  Cloning of human Dab1 and sequence determinations suggest a 96% identity to the mouse sequence.  Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins.  Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2.  In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.

Product Details

Anti-Dab1 (400-555) (RABBIT) Antibody - 100-401-225
rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody
Rabbit
Polyclonal
Antiserum

Target Details

Dab1  - View All Dab1 Products
Mouse
Conjugated Peptide
This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.
Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.  This antibody is directed against Dab1 from mouse.  Cross-reactivity with other species has not been determined.  No reaction occurs with human or mouse Dab2.
UniProtKB - P97318
NCBI - NP_034144.1
GeneID - 13131

Application Details

IHC, WB
Biochemical Assay, IF, Multiplex  - View References
Anti-Dab1 antibody has been tested by western blot and immunohistochemistry and is suitable for the detection of Dab1 by immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate.  For western blotting block the blot using 5% BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1% BLOTTO in TTBS for 1 h at room temperature.  For immunoprecipitation use 1 µl of antiserum per 500 µg of brain lysate.  Perform immunoprecipitation at 4°C for 2 h.  For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1% (v/v) NP-40, 10 µg/ml aprotinin and 10 µg/ml leupeptin.

Formulation

Liquid (sterile filtered)
75 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). The E3 Ubiquitin Ligase CRL5 Regulates Dentate Gyrus Morphogenesis, Adult Neurogenesis, and Animal Behavior. Frontiers in Neuroscience
Applications
WB, IB, PCA
(). Serine 1283 in extracellular matrix glycoprotein Reelin is crucial for Reelin’s function in brain development. bioRxiv Preprint
Applications
WB, IB, PCA
(). RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion. Development
Applications
Biochemistry
(). Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin. eNeuro
Applications
WB, IB, PCA
(). Localization of the paranodal protein Caspr in the mammalian retina. Mol Vis
Applications
WB, IB, PCA
(). Weight increase and overweight are associated with DNA oxidative damage in skeletal muscle. Clin Nutr.
Applications
IHC, ICC, Histology
(). The transcription factor Bhlhb4 is required for rod bipolar cell maturation. Neuron
Applications
IF, Confocal Microscopy
(). Probing inner retinal circuits in the rod pathway: a comparison of c-fos activation in mutant mice. Vis Neurosci.
Applications
IHC, ICC, Histology; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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