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            Choline Oxidase Antibody
Goat Polyclonal
            
                200-101-094
                200-101-094S
                200-101-094-0100
        
        
            
                50 mg
                25 µL
                100 µg
        
            
                
                    Lyophilized
                    Liquid (sterile filtered)
                    Lyophilized
            
            
                
                WB
            
                    
                
                Alcaligenes sp.
            
                            
                
                 Goat
            
                
            
        
        Shipping info:
        
    $50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
                    
                    
Anti-Choline Oxidase (Alcaligenes species) (GOAT) Antibody (BULK ORDER) - 200-101-094                    
                
                
                    
                    
goat anti-Choline Oxidase Antibody, codA antibody                    
                
                
                    
                    
Goat                    
                
                
                    
                    
Polyclonal                    
                
                
                    
                    
IgG                    
                
            Target Details
                    
                    
CHOX_ALCSP                         - View All CHOX_ALCSP Products
                            
                    
                
                
                    
                    
Alcaligenes sp.                                            
                
                
                    
                    
Native Protein                                            
                
                
                    
                    
Choline Oxidase [Alcaligenes species]                                            
                
                
                    
                    
Anti-Choline Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].  Cross reactivity against Choline Oxidase from other sources is unknown.                                            
                
                
                Application Details
                        
                        
WB
                        
                    
                    
                        
                        
Choline Oxidase Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Choline Oxidase [Alcaligenes species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:22,000 of the reconstitution concentration is suggested for this product.
                        
                    
                                                    Formulation
                        
                        
10.0 mg/mL by UV absorbance at 280 nm                        
                    
                    
                        
                        
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2                        
                    
                    
                        
                        
0.01% (w/v) Sodium Azide                        
                    
                    
                        
                        
None                        
                    
                    
                        
                        
5.0 mL                        
                    
                    
                        
                        
Restore with deionized water (or equivalent)                        
                    
            Shipping & Handling
                    
                    
Ambient                    
                
                
                    
                    
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.                     
                
                
                    
                    
Expiration date is one (1) year from date of receipt.                    
                
        
                Choline Oxidase catalytic activity is Choline + 2 O2 + H2O = betaine + 2 H2O2.  This protein is involved in step 1 of the subpathway that synthesizes betaine from choline.
            
        
            This product is for research use only and is not intended for therapeutic or diagnostic applications.  Please contact a technical service representative for more information.  All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin.  Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.  All properties listed are typical characteristics and are not specifications.  All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control.  All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale.  Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc.  If you require a commercial license to use this material and do not have one, then return this material, unopened to:  Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.