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Datasheet

Chloramphenicol-Acetyl Transferase Antibody

Rabbit Polyclonal

100-401-463
100 µL
ELISA
Cat
Rabbit
$275.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Chloramphenicol acetyltransferase enzyme is an effector of chloramphenicol resistance in bacteria.

Product Details

Anti-Chloramphenicol-Acetyl Transferase (CAT) (RABBIT) Antibody - 100-401-463
Rabbit Anti-Chloramphenicol-Acetyl Transferase Antibody, anti-CAT antibody, Rabbit Anti Chloramphenicol-Acetyl Transferase, rabbit anti CAT
Rabbit
Polyclonal
Antiserum

Target Details

Cat
Conjugated Peptide
This antiserum was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near a 30-60 of bacterial chloramphenicol acetyl transferase (CAT). This antibody identifies recombinant CAT as a predominant band of 26 kDa in eukaryotic cells transfected with a plasmid bearing the CAT gene.
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified chloramphenicol acetyl transferase (CAT). Cross reactivity against chloramphenicol acetyl transferase from other tissues and species may occur but have not been specifically determined.

Application Details

ELISA
Anti-Chloramphenicol-Acetyl Transferase Antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Formulation

Lyophilized
85 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.