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Datasheet

CD27 Phycoerythrin Antibody

Mouse Monoclonal O323 IgG1 kappa

200-308-N65
500 µL
Liquid (sterile filtered)
FC
Human
Mouse
$389.00 /Per Item
Availability: Ships in approximately 14 days
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Background

CD27 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The CD27 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naïve vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta.

Product Details

Anti-CD27 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N65
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7
Mouse
R-Phycoerythrin (RPE)
Monoclonal
IgG1

Target Details

CD27  - View All CD27 Products
Human
Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.
Phycoerythrin conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27. Reactivity is observed against human CD27, Baboon, Cynomolgus, and Rhesus. Cross reactivity with CD27 from other sources has not been tested. Anti-CD27 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
P26842 - UniProtKB
NP_001233.1 - NCBI Protein
939 - Gene ID

Application Details

FC
Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Formulation

0.05 mg/mL Sufficient to run approximately 100 tests
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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