BORIS Antibody
Anti-BORIS Antibody - Western Blot
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Datasheet

BORIS Antibody

Rabbit Polyclonal

600-401-907
100 µg
ELISA, WB, ChIP, IF
Human
Rabbit
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$399.00 /Per Item
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Description

Background

Anti-BORIS antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. BORIS (Brother of the Regulator of Imprinted Sites) also known as  CCCTC-binding factor-like protein, is  normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development.  However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers.  Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation.  BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line.  BORIS is found in both the nucleus and cytoplasm. BORIS antibody can be used to investigate epigenetic regulation.

Application Note

BORIS affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.

Purity/Specificity

This affinity-purified antibody is directed against the human BORIS protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest cross reactivity with BORIS proteins from human and chimpanzee.  Partial reactivity may occur against BORIS from mouse, dog and rat sources based on varying degrees of homology to the immunizing sequence.  Reactivity against homologues from other sources is not known.

Anti-BORIS (RABBIT) Antibody - 600-401-907
rabbit anti-BORIS Antibody, Transcriptional repressor CTCFL, Brother of the regulator of imprinted sites antibody, CCCTC binding factor (zinc finger protein) like antibody, CCCTC-binding factor antibody, CTCF paralog antibody, CTCF-like, cancer/testis antigen 27, CT27, CTCF-T
CTCFL
ELISA, WB
ChIP, IF
Human
Human
Rabbit
Polyclonal
IgG
Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein.
Liquid (sterile filtered)
1.28 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Dry Ice
Store BORIS Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.
Janssen SM et al. BORIS/CTCFL promotes a switch from a proliferative towards an invasive phenotype in melanoma cells. Cell Death Discov. (2020)
Applications
WB, IB, PCA
Macaluso M et al. Integrating role of T antigen, Rb2/p130, CTCF and BORIS in mediating non-canonical endoplasmic reticulum-dependent death pathways triggered by chronic ER stress in mouse medulloblastoma. Cell Cycle. (2012)
Applications
ChIP
Rosa-Garrido M et al. A cell cycle role for the epigenetic factor CTCF-L/BORIS. PLoS One. (2012)
Applications
IF, Confocal Microscopy; WB, IB, PCA
Fiorentino FP et al. CTCF and BORIS regulate Rb2/p130 gene transcription: a novel mechanism and a new paradigm for understanding the biology of lung cancer. Mol Cancer Res. (2011)
Applications
ChIP
Hines WC et al. BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas. PLoS One. (2010)
Applications
WB, IB, PCA
Dougherty CJ et al. Selective apoptosis of breast cancer cells by siRNA targeting of BORIS. Biochem Biophys Res Commun. (2008)
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.