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Biotin Antibody
Goat Polyclonal
3 References
600-101-098
1 mg
Liquid (sterile filtered)
ELISA, IF
Biotin
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Biotin (GOAT) Antibody - 600-101-098
goat anti biotin antibody
Goat
Polyclonal
IgG
Target Details
Biotin
Other
Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.
Application Details
ELISA
IF
- View References
Anti-Biotin has been tested by ELISA is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Optimal concentrations in other immunoassays should be determined by the researcher.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
This product is stable for several weeks at 4° C as an undiluted liquid. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
Sarmento et al. (2018). Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures. Nature Communications
Applications
IF, Confocal Microscopy
Liu C et al. (2015). Simultaneous detection of multiple proteins that bind to the identical ligand in supported lipid bilayers. Anal Chem.
Applications
Fluorescent Electrophoregrams
Vieira N et al. (2014). SNX31: a novel sorting nexin associated with the uroplakin-degrading multivesicular bodies in terminally differentiated urothelial cells. PloS One
Applications
IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.