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Datasheet

Beta-Phosphoglucomutase Antibody Peroxidase Conjugated

Goat Polyclonal

200-103-237 200-103-237S 200-103-237-0100
20 mg 25 µL 100 µg
Lactococcus lacti
Goat
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Background

Beta-Phosphoglucomutase catalyzes the interconversion of D-glucose 1-phosphate and D-glucose 6-phosphate, forming beta-D-glucose 1,6-(bis)phosphate as an intermediate. The beta-phosphoglucomutase acts on the beta-C1 anomer of G1P. Glucose or lactose are used in preference to maltose, which is only utilized after glucose or lactose has been exhausted. It plays a key role in the regulation of the flow of carbohydrate intermediates in glycolysis and the formation of the sugar nucleotide UDP-glucose. This enzyme belongs to the family of isomerases, specifically the phosphotransferases (phosphomutases), which transfer phosphate groups within a molecule. The systematic name of this enzyme class is beta-D-glucose 1,6-phosphomutase. This enzyme participates in starch and sucrose metabolism.

Product Details

Anti-Beta Phosphoglucomutase (GOAT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-103-237
goat anti-Beta-Phosphoglucomutase Antibody HRP Conjugation, peroxidase conjugated goat anti-Beta PGM antibody, Glucose phosphomutase 1 antibody, Glucose phosphomutase antibody, PGM antibody, pgmB antibody
Goat
Peroxidase (HRP)
Polyclonal
IgG

Target Details

pgmB  - View All pgmB Products
Lactococcus lacti
Native Protein
Beta Phosphoglucomutase [Lactococcus lacti]
Beta-Phosphoglucomutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified b -Phosphoglucomutase [Lactococcus lacti].  Cross reactivity against b -Phosphoglucomutase from other sources is unknown.

Application Details

Anti-Beta-Phosphoglucomutase has been assayed against 1.0 ug of b-Phosphoglucomutase [Lactococcus lacti] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:20,000 is suggested for this product.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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