Beta Galactosidase Antibody
Anti-B-Galactosidase Antibody - Western Blot
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Datasheet

Beta Galactosidase Antibody

Rabbit Polyclonal

9 References
100-4136
2 mL
WB, IHC, IF, Multiplex
b-GAL
Rabbit
$275.00 /Per Item
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Background

Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.

Product Details

Anti-Beta Galactosidase (E. coli) (RABBIT) Antibody - 100-4136
rabbit anti-BETA GALACTOSIDASE Antibody, beta-galactosidase, rabbit anti-beta-gal antibody
Rabbit
Polyclonal
Antiserum

Target Details

LacZ  - View All LacZ Products
b-GAL
Native Protein
Beta Galactosidase (E.coli)
Beta-Galactosidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Beta Galactosidase [E. coli].  Cross reactivity against Beta Galactosidase from other sources may occur but have not been specifically determined.
UniProtKB - P00722
NCBI - NP_414878.1

Application Details

WB
IF, IHC, Multiplex  - View References
Anti-Beta-Gal Antibody has been tested by western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Formulation

Lyophilized
85 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Development of Novel Heparin/Protamine Nanoparticles Useful for Delivery of Exogenous Proteins In Vitro and In Vivo. Nanomaterials (Basel).
Applications
IHC, ICC, Histology
(). Hippo signaling promotes lung epithelial lineage commitment by curbing Fgf10 and β-catenin signaling. Development.
Applications
IF, Confocal Microscopy
(). Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency. Journal of Virology
Applications
IF, Confocal Microscopy; Multiplex Assay
(). Fgf10-Hippo Epithelial-Mesenchymal Crosstalk Maintains and Recruits Lung Basal Stem Cells. Developmental Cell
Applications
IHC, ICC, Histology
(). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis Cell Stem Cell.
Applications
IF, Confocal Microscopy
(). Herpes Simplex Virus and Interferon Signaling Induce Novel Autophagic Clusters in Sensory Neurons. Journal of Virology
Applications
IHC, ICC, Histology; Multiplex Assay
(). Cooperation between both Wnt/β-catenin and PTEN/PI3K/Akt signaling promotes primitive hematopoietic stem cell self-renewal and expansion. Genes Dev.
Applications
IF, Confocal Microscopy
(). Peptide mass fingerprinting by matrix‐assisted laser desorption ionization mass spectrometry of proteins detected by immunostaining on nitrocellulose. Proteomics.
Applications
WB, IB, PCA
(). Hoxb13 is required for normal differentiation and secretory function of the ventral prostate. Development.
Applications
IF, Confocal Microscopy

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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