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Beta Actin Antibody
Rabbit Polyclonal
47 References
600-401-886S
600-401-886
25 µL
200 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA, IF
Human, Mouse, Leopard Frog
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Beta Actin (RABBIT) Antibody - 600-401-886
rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
Rabbit
Polyclonal
IgG
Target Details
ACTB - View All ACTB Products
Human, Mouse, Leopard Frog
Conjugated Peptide
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Application Details
ELISA, IF, WB
Anti-beta Actin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Low R et al. (2023). Prolonged Inhibition of the MEK1/2-ERK Signaling Axis Primes Interleukin-1 Beta Expression through Histone 3 Lysine 9 Demethylation in Murine Macrophages. Int J Mol Sci.
Applications
WB, IB, PCA
McKamey SG et al. (2022). Antagonism of the transient receptor potential melastatin‑2 channel leads to targeted antitumor effects in primary human malignant melanoma cells. Int J Oncol.
Applications
WB, IB, PCA
Hildebrandt J et al. (2022). Pro-inflammatory activation changes intracellular transport of bevacizumab in the retinal pigment epithelium in vitro. Graefes Arch Clin Exp Ophthalmol.
Applications
WB, IB, PCA
Rodrigues-Amorim D et al. (2021). The Role of the Second Extracellular Loop of Norepinephrine Transporter, Neurotrophin-3 and Tropomyosin Receptor Kinase C in T Cells: A Peripheral Biomarker in the Etiology of Schizophrenia. Int J Mol Sci.
Applications
WB, IB, PCA
Ilic N et al. (2021). SMURF2‐mediated ubiquitin signaling plays an essential role in the regulation of PARP1 PARylating activity, molecular interactions, and functions in mammalian cells. FASEB J.
Applications
Undefined
Ogi K et al. (2021). ORMDL3 overexpression facilitates FcεRI-mediated transcription of proinflammatory cytokines and thapsigargin-mediated PERK phosphorylation in RBL-2H3 cells. Immun Inflamm Dis.
Applications
WB, IB, PCA
Rosas-Ballina M. et al. (2020). Classical Activation of Macrophages Leads to Lipid Droplet Formation Without de novo Fatty Acid Synthesis. Front Immunol.
Applications
WB, IB, PCA
Tatomir A et al. (2020). RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis. Frontiers in Immunology
Applications
WB, IB, PCA
D'Souza M et al. (2019). Regulator of G-protein signaling 5 protein protects against anxiety-and depression-like behavior. Behav Pharmacol.
Applications
WB, IB, PCA
Coleman et al. (2018). Cathepsin B plays a key role in optimal production of the influenza A virus. Journal of Virology & Antiviral Research
Applications
WB, IB, PCA
Borroni et al. (2018). Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin. Aging Cell
Applications
WB, IB, PCA
Olive et al. (2018). Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies. PLOS One
Applications
WB, IB, PCA
Dai J et al. (2018). Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation. J Immunol.
Applications
WB, IB, PCA
Gillis NE et al. (2018). Thyroid Hormone Receptor β Suppression of RUNX2 Is Mediated by Brahma-Related Gene 1–Dependent Chromatin Remodeling. Endocrinology.
Applications
WB, IB, PCA
Chen et al. (2017). Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice. Oncology Letters
Applications
WB, IB, PCA
Lodola et al. (2017). VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells. Oncotarget
Applications
WB, IB, PCA
Schanze N et al. (2017). 3-Iodothyronamine decreases expression of genes involved in iodide metabolism in mouse thyroids and inhibits iodide uptake in PCCL3 thyrocytes. Thyroid.
Applications
WB, IB, PCA
Campos et al. (2016). Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nature Communications
Applications
WB, IB, PCA
Carr FE et al. (2016). Thyroid hormone receptor-β (TRβ) mediates runt-related transcription factor 2 (Runx2) expression in thyroid cancer cells: A novel signaling pathway in thyroid cancer. Endocrinology.
Applications
WB, IB, PCA
Zuccolo E et al. (2016). Constitutive Store-Operated Ca(2+) Entry Leads to Enhanced Nitric Oxide Production and Proliferation in Infantile Hemangioma-Derived Endothelial Colony-Forming Cells. Stem Cells Dev.
Applications
WB, IB, PCA
Hopkins et al. (2015). Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells. International Journal of Oncology
Applications
WB, IB, PCA
Koh et al. (2015). Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncology Reports
Applications
WB, IB, PCA
Rae et al. (2015). A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis. Oncotarget
Applications
WB, IB, PCA
Maragkoudaki et al. (2015). Specific detection of OCT4 isoforms in inflammatory bowel disease. Gut Pathogens
Applications
WB, IB, PCA
Dragoni S et al. (2014). Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis. PLoS One.
Applications
WB, IB, PCA
Nelson PT et al. (2014). ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. Acta Neuropathol.
Applications
WB, IB, PCA
Papaconstantinou I et al. (2014). Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats. Int J Surg.
Applications
WB, IB, PCA
Dragoni S et al. (2014). Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma. Biomed Res Int.
Applications
WB, IB, PCA
Zha Y et al. (2014). MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression. Cell Death Dis.
Applications
WB, IB, PCA
Kjellberg, MA et al. (2014). Alternation in the glycolipid transfer protein expression causes changes in the cellular lipidome. PloS One
Applications
WB, IB, PCA
Muppidi, A et al. (2014). Targeted delivery of ubiquitin-conjugated BH3 peptide-based Mcl-1 inhibitors into cancer cells. Bioconjugate Chemistry
Applications
WB, IB, PCA
Laforenza U et al. (2013). Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes. PLoS One
Applications
WB, IB, PCA
Dragoni S et al. (2013). Canonical Transient Receptor Potential 3 Channel Triggers Vascular Endothelial Growth Factor-Induced Intracellular Ca2+ Oscillations in Endothelial Progenitor …. Stem Cells Dev.
Applications
WB, IB, PCA
Wang X et al. (2013). HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation. BMC Genomics.
Applications
WB, IB, PCA
Kjellberg, MA et al. (2013). Glycolipid transfer protein expression is affected by glycosphingolipid synthesis. PloS One
Applications
WB, IB, PCA
Mao L et al. (2012). Cyclin E1 is a common target of BMI1 and MYCN and a prognostic marker for neuroblastoma progression. Oncogene.
Applications
WB, IB, PCA
Zha Y et al. (2012). Functional dissection of HOXD cluster genes in regulation of neuroblastoma cell proliferation and differentiation. PLoS One
Applications
WB, IB, PCA
Lodola F et al. (2012). Store-operated Ca 2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients. PLoS One
Applications
WB, IB, PCA
Zhu B et al. (2012). Peroxisome proliferator-activated receptor β/δ cross talks with E2F and attenuates mitosis in HRAS-expressing cells. Mol Cell Biol.
Applications
WB, IB, PCA
Molhoek KR et al. (2011). Comprehensive analysis of RTK activation in human melanomas reveals autocrine signaling through IGF-1R. Melanoma Res.
Applications
WB, IB, PCA
Dragoni S et al. (2011). Vascular endothelial growth factor stimulates endothelial colony forming cells proliferation and tubulogenesis by inducing oscillations in intracellular Ca2+ concentration. Stem Cells.
Applications
WB, IB, PCA
Choi LJ et al. (2010). Coordinate Down-regulation of Adenylyl Cyclase Isoforms and the Stimulatory G Protein (Gs) in Intestinal Epithelial Cell Differentiation. J Biol Chem.
Applications
WB, IB, PCA
Yang L et al. (2010). Loss of negative feedback control of nuclear factor-κB2 activity in lymphocytes leads to fatal lung inflammation. Am J Pathol.
Applications
WB, IB, PCA
Calva-Cerqueira D et al. (2010). Discovery of the BMPR1A promoter and germline mutations that cause juvenile polyposis. Hum Mol Genet.
Applications
WB, IB, PCA
Khoh-Reiter S et al. (2009). Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model. BMC Ophthalmol.
Applications
WB, IB, PCA
Lee W et al. (2008). Olfactory sensory neuron‐specific and sexually dimorphic expression of protocadherin 20. J Comp Neurol.
Applications
WB, IB, PCA
Sweeney Jr. WE et al. (2008). Src inhibition ameliorates polycystic kidney disease. J Am Soc Nephrol.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
