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Datasheet

Beta Actin Antibody

Chicken Polyclonal

200-901-Z55
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human, Mouse, Rat
Chicken
$475.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Product Details

Anti-Beta Actin (CHICKEN) Antibody - 200-901-Z55
Beta-actin Antibody, BRWS1, PS1TP5BP1, Actin, cytoplasmic 1, Beta-actin
Chicken
Polyclonal
IgY

Target Details

ACTB  - View All ACTB Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Beta-actin antibody was prepared from chicken egg fractions produced by repeated immunizations with a 16 amino acid synthetic peptide from near the N-terminus of human beta-actin.
Anti-Beta-actin Antibody is an IgY fraction that has been affinity purified by immunoaffinity purification. Cross reactivity with Beta-actin from other sources has not been determined.
P60709 - UniProtKB
AAH02409 - NCBI Protein
60 - Gene ID

Application Details

ELISA, IHC, WB
Anti-Beta-actin Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 42 kDa in Western Blots of specific cell lysates and tissues.

Formulation

1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.