Datasheet

APG7 Antibody

Name: APG7 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

7 References

Item No. 600-401-487

Size: 100 µg

Applications: WB, ELISA

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Background

Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.

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Specifications for APG7 Antibody

Product Details

Description: Anti-APG7 (RABBIT) Antibody - 600-401-487

Synonyms: rabbit anti-APG7 Antibody, APG-7, APG 7, APG7 autophagy 7-like antibody, APG7 like antibody, APG7L antibody, ATG 7 antibody, ATG-7 antibody, ATG7 autophagy related 7 homolog antibody

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: ATG7 - View All ATG7 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 550-575 of Human Apg7 protein.

Purity/Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100% homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100% identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.

Database Links:

UniProtKB - O95352

NCBI - NP_001129503.2

GeneID - 10533

Application Details

Tested Applications: ELISA, WB

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 78 kDa in size corresponding to human Apg7 by western blotting in the appropriate cell lysate or extract. Human Apg7 has been reported to form a homodimer at approximately 160 kDa.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (7)

Fattah et al. (2015). Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency. Journal of Immunology

Applications

WB, IB, PCA

PubMed

Bhattacharya A et al. (2014). Deficiency of autophagy in dendritic cells protects against experimental autoimmune encephalomyelitis. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Shin JN et al. (2013). Inflammasome activation by altered proteostasis. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Fung C et al. (2012). EGFR tyrosine kinase inhibition induces autophagy in cancer cells. Cancer Biol Ther.

Applications

WB, IB, PCA

PubMed

Liu XD et al. (2012). Transient aggregation of ubiquitinated proteins is a cytosolic unfolded protein response to inflammation and endoplasmic reticulum stress. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Sarkar S et al. (2007). Small molecules enhance autophagy and reduce toxicity in Huntington's disease models. Nat Chem Biol.

Applications

WB, IB, PCA

PubMed

Sarkar S et al. (2007). Trehalose, a novel mTOR-independent autophagy enhancer, accelerates the clearance of mutant huntingtin and α-synuclein. J Biol Chem.

Applications

WB, IB, PCA

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.