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Datasheet

Apelin Antibody

Rabbit Polyclonal

600-401-R09
100 µg
WB
Human, Mouse, Rat
Rabbit
$445.00 /Per Item
Availability: Ships in approximately 14 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei that acts on specific receptors located on vasopressinergic neurons. The apelin gene to Xq25-q26.3 based on sequence similarity between the apelin sequence and a chromosome X PAC clone. This gene encodes a peptide that functions as an endogenous ligand for the G protein coupled receptor APJ. The encoded protein is synthesized as a prepropeptide that is processed into biologically active C-terminal fragments. The peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. This antibody is suitable for researchers interested in neuroscience research.

Product Details

Anti-Apelin (RABBIT) Antibody - 600-401-R09
APJ endogenous ligand,
Rabbit
Polyclonal
IgG

Target Details

APLN  - View All APLN Products
Human, Mouse, Rat
Conjugated Peptide
Apelin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Apelin.
Anti-Apelin antibody is directed against human Apelin protein.  The product was affinity purified from monospecific antiserum by immunoaffinity purification.  A BLAST analysis was used to suggest reactivity with this protein from human, mouse, and rat based on 100% homology for the immunogen sequence.  Cross reactivity with Apelin from other sources has not been determined.

Application Details

WB
Anti-Apelin is tested for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~8.6 kDa corresponding to the appropriate cell lysate or extract.

Formulation

0.5 mg/mL by UV absorbance at 280 nm
0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
0.05mg Thimerosal
5mg BSA
200µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.