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Datasheet

Anti-Acetylcholinesterase Antibody Peroxidase Conjugated

Goat Polyclonal

200-103-232 200-103-232S 200-103-232-0100
20 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
WB
Bovine
Goat
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$125.00 /Per Item
$455.00 /Per Item
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Background

Acetylcholinesterase, or AChE, is an important protease that terminates the transmission of the neurotransmitter, acetylcholine, by hydrolysis. Hydrolysis by AChE on ACh prevents excessive neural firing between adjacent nerves. Horse Radish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody is ideal for investigators in Signal Transduction, Cell Signaling, Cardiovascular , and Immunology research.

Product Details

Anti-Acetylcholinesterase (GOAT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-103-232
goat anti-Acetyl Cholinesterase Antibody, HRP conjugated goat anti-Acetyl Cholinesterase Antibody, Acetylcholinesterase, AChE, HRP, Horseradish peroxidase, HRP, Horseradish peroxidase
Goat
Peroxidase (HRP)
Polyclonal
IgG

Target Details

ACHE  - View All ACHE Products
Bovine
Native Protein
Acetylcholinesterase [Bovine Erythrocytes]
Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Acetylcholinesterase [Bovine Erythrocytes].  Cross reactivity against Acetylcholinesterase from other sources is unknown.

Application Details

WB
Anti-Acetylcholinesterase (Bovine Erythrocytes) antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Acetylcholinesterase [Bovine Erythrocytes] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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