Alpha Internexin Antibody
Western Blot of Anti-Alpha Internexin (Mouse) Antibody - 200-301-D04
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Datasheet

Alpha Internexin Antibody

Mouse Monoclonal 1D2 IgG1

900-301-D04
100 µL
IHC, WB
Human, Mouse, Rat
Mouse
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$411.00 /Per Item
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Description

Background

Anti-Alpha Internexin antibody recognizes alpha-internexin which is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits. Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals. Anti-Alpha Internexin antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Application Note

Anti-Alpha Internexin antibody is tested for use in ELISA, Western Blotting, IHC and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 66kDa in size corresponding to alpha internexin proteins in the appropriate cell lysate or extract. Anti-Alpha Internexin antibody can be used on formalin-fixed cells in tissue culture, cryostat sections. The epitope recognized by the ID2 clone is in the C-terminal non-helical extension of the protein and is unusually resistant to aldehyde fixation, so that this antibody is ideal for studies of paraffin embedded formalin fixed histological sections.

Purity/Specificity

Anti-Alpha Internexin Antibody is directed against rat Alpha Internexin. The antibody is protein G purified from cell culture supernatant. Reactivity is expected against human and mouse. Cross reactivity from other sources has not been determined.

Anti-Alpha Internexin (Mouse) Antibody - 900-301-D04
internexin, NF-66, NEF5, Alpha-internexin, Alpha-Inx, Ina, Inexa
Ina
IHC, WB
Rat
Human, Mouse, Rat
Mouse
Monoclonal
1D2
IgG1
Recombinant Protein
Anti-Alpha Internexin Antibody was produced by repeated immunizations with recombinant rat alpha internexin expressed in E. coli.
Liquid
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Dry Ice
Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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