AKT2 Western Chemiluminescent Blotting Kit
AKT2 Antibody - Western Blot
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Datasheet

AKT2 Western Chemiluminescent Blotting Kit

Rat Monoclonal 16G11.E8 IgG2a kappa

KCAE71
1 Kit
WB
Human
Rat
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Description

Background

Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT2 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT2 protein. AKT2 Western Bloting kit allows for the detection of endogenous protein levels of human AKT2 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT2. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT2 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT2 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Application Note

Use Rockland Immunochemicals’ Anti-AKT2 Chemiluminescent Kit for Western Blotting for detection of human AKT2 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT2 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Purity/Specificity

The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT1 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Storage Condition

See kit insert for complete instructions.

AKT2 Western Blot Kit - KCAE71
AKT 2 western blotting, AKT-2, AKT 2 antibody, AKT-2, PKB antibody, PKB beta antibody, PKBBETA antibody, PRKBB antibody, Protein kinase Akt 2 antibody, Protein kinase B beta antibody, RAC-beta serine/threonine-protein kinase, RAC-PK-beta, western bloting kit, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit
AKT2
WB
Human
Human
Rat
Monoclonal
16G11.E8
IgG2a
Chemiluminescent Western Blot Kit
Dry Ice
See kit insert for complete instructions.
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
None

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This material is subject to proprietary rights and is sold under license from ROCKLAND IMMUNOCHEMICALS Inc. This product is licensed for sale only for 'research-use' only. There is no implied license hereunder for any commercial use. FemtoMax® is a trademark of ROCKLAND IMMUNOCHEMICALS Inc. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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