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6X His Tag Antibody DyLight™ 649
Mouse Monoclonal 33D10.D2.G8 IgG1 kappa
5 References
200-343-382
100 µg
Lyophilized
WB, ELISA, Dot Blot, Biochemical Assay, Microarray
His-Tag
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-6X His Epitope Tag (MOUSE) Monoclonal Antibody DyLight™ 649 Conjugated - 200-343-382
mouse anti-6X His Tag DyLight™ 649 conjugated Antibody, DyLight™ 649 conjugated mouse anti-6X His Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Mouse
DyLight™ 649
Monoclonal
IgG1
Target Details
His-Tag
Conjugated Peptide
This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
This protein-A purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Application Details
Dot Blot, ELISA, WB
Biochemical Assay, Microarray
- View References
Anti-6X HIS tagged Antibody conjugated to DyLight™649 has been tested by ELISA, dot blot, and western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA), fluorescent polarization, FRET and fluorescent western blotting. Anti-6X HIS Antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Daguer JP et al. (2021). Dual Bcl-XL/Bcl-2 inhibitors discovered from DNA-encoded libraries using a fragment pairing strategy. Bioorg Med Chem.
Applications
Microarray, Microarray chip
Hunter SA et al. (2021). An engineered ligand trap inhibits leukemia inhibitory factor as pancreatic cancer treatment strategy. Commun Biol.
Applications
Kinetic Exclusion Assay
Mehta N et al. (2020). An engineered antibody binds a distinct epitope and is a potent inhibitor of murine and human VISTA. Sci Rep.
Applications
Kinetic Exclusion Assay
Kariolis et al. (2017). Inhibition of the GAS6/AXL pathway augments the efficacy of chemotherapies. Journal of Clinical Investigation
Applications
Kinetic Exclusion Assay
Kariolis MS et al. (2014). An engineered Axl'decoy receptor'effectively silences the Gas6-Axl signaling axis. Nat Chem Biol.
Applications
Kinetic Exclusion Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.