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TMB Substrate for WB MaxTag™

100 mL
$139.00 /Per Item
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This following method is suggested as a guideline for the use for Western Blot with nitrocellulose or PVDF membranes. The TMB Substrate in 100 mL bottle TMBM-101 and the TMB Precipitating Agent in 10 mL bottle TMBM-102 are used together. Mix 9 ml of the TMB Substrate and 1.0 ml of the TMB Precipitating Agent and mix thoroughly. The substrate is now ready to use. Be certain to use within 24 hours. After antibody incubations and wash, aspirate the wash solution and add approximately 5 ml of freshly prepared substrate solution. Incubate until color develops (usually 2 to 20 min). Wash the membrane with 2 changes of deionized water for 5 min each.

Product Details

TMB Substrate for Western Blot MaxTag™ use with 10 ml of TMB Substrate Precipitating Agent (p/n TMBM-102). See kit insert for details. - TMBM-101
3,3’,5,5’-Tetramethylbenzidine, TMB Membrane Peroxidase Substrate, TMB Membrane HRP Substrate
Colorimetric Western Blot Kit

Shipping & Handling

See kit insert for complete instructions.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.