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Datasheet Protocol

Small Sample Targeted ChIP Kit

1 Kit
This product is discontinued


Association between proteins and DNA is a major mechanism in many vital cellular functions such as gene transcription and epigenetic silencing. It is crucial to understand these interactions and the mechanisms by which they control and guide gene regulation pathways and cellular proliferation. Chromatin immunoprecipitation (ChIP) is a technique to analyze the association of proteins with specific genomic regions in intact cells. ChIP can be used to study changes in epigenetic signatures, chromatin remodelling and transcription regulator recruitment to specific genomic sites. In ChIP, living cells are first fixed with a reversible crosslinking agent to stabilize protein-DNA interactions. The most widely used reagent to fix cells is formaldehyde which generates covalent bonds between amino or imino groups of proteins and nucleic acids. Formaldehyde treatment crosslinks both DNA-protein as well as protein-protein complexes. However conventional ChIP protocols require high numbers of cells (hundreds of thousands cells at least) limiting the application for ChIP technology to few cell samples. More recently, ChIP assays on smallest amount of cells have been reported. Nevertheless the procedure requires tedious optimization of several reaction conditions to face the increased background observed in ChIP performed with reduced amount of cells. That might consequently lead to considerable time and lab expenditures. To reduce these tedious steps, Small Sample Targeted ChIP Kit has optimized reagents and protocol to enable successful ChIP on as few as 10 000 cells. Moreover, the Small Sample Targeted ChIP Kit protocol has been thoroughly optimized for ChIP followed by high-throughput sequencing on Illumina® Next-Gen sequencers.

Product Details

Small Sample Targeted ChIP Kit - KOA0886
ChIP assay, cross-linking, chromatin, Chromatin Immunoprecipitation, micro ChIP
ChIP Kit or Chromatin Immunoprecipitation Kit

Application Details

The different steps of the ChIP assay are cell fixation (crosslinking), chromatin shearing, immunoprecipitation, reverse crosslinking followed by DNA purification and analysis of the immunoprecipitated DNA. Suitable for 10 reactions.

Shipping & Handling

Dry Ice
See kit insert for components stored at 2-8°C and -20°C. Do not freeze Protein A and magnetic beads.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.