Sheep Hemagglutination Kit
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Background
Hemagglutination method is used for titer evaluation of viruses (e.g. Influenza, COVID-19 etc.), bacteria (e.g. Staphylococci, Shigella etc.), proteins (e.g. certain glycoproteins such as Lectins) and other agents which have the ability to attach to red blood cells (RBC)’s surface molecules. For example, the influenza viruses agglutinate RBCs through their interaction with sialic acid receptors on the host cells. At a molecular level, viral hemagglutinins bind to sialic acid linked to galactose residues through alpha 2.3 or 2.6 linkage, which varies from species to species. Once a hemagglutinin agglutinates the RBCs, each of the agglutinating molecule attaches to RBCs to form a lattice-structure or a meshwork. This prevents RBCs from settling down from suspension in a round bottom 96 well plate. Visually, a hemagglutinated sample will generate a uniform red colored suspension while a sample lacking hemagglutination will lead to settling down of RBCs to the bottom of wells to form button or dot shape. In viral hemagglutination assay, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.05% -0.1%) for 30 minutes to an hour at room temperature or 4° C. Thereafter, lattice formation is observed and the titer of a hemagglutination assay can be determined by the last viable "lattice" structure found. If diluted further, the amount of Virus particles will be less than the RBCs and thus not be able to agglutinate them. A Hemagglutination-inhibition (HAI) assay will involve titration of the viral hemagglutination with an anti-viral antibody (often from serum of human or animal infected with that virus) for inhibition of hemagglutination (i.e. neutralization of virus). HAI is one of the most commonly used methods to quantify immunity from influenza and other respiratory viral disease vaccines and is considered the gold standard as a correlate of vaccination mediated protection.
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.