Rat MCP-1 - CCL2 ELISA Kit
KOA0542
1 Kit
ELISA
Rat
15.6 pg/ml - 1000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rat MCP-1/CCL2 AccuSignal ELISA Kit - KOA0542
C-C motif chemokine 2, CCL2, CCL2_HUMAN, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, Monocyte chemoattractant protein 1, Monocyte chemoattractant protein-1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je), Small inducible cytokine subfamily A (Cys-Cys), member 2, Small-inducible cytokine A2, SMC-CF
ELISA Kit
15.6 pg/ml - 1000 pg/ml
Target Details
CCL2 - View All CCL2 Products
Rat
Expression system for standard: NSO; Immunogen sequence: Q24-N148
Natural and recombinant rat MCP-1. There is no detectable cross-reactivity with other relevant proteins.
P14844 - UniProtKB
NP_113718.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat MCP-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates or serum to each empty well. It is recommended that each rat MCP-1 standard solution and each sample be measured in duplicate.
Formulation
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Monocyte chemoattractant protein-1(MCP-1), a member of the chemokine(chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3
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