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Datasheet Protocol

Rat DKK1 ELISA Kit

KOA0865
1 Kit
ELISA
Rat
46.9 pg/ml - 3000 pg/ml
$589.00 /Per Item
Out of stock
Ships in approximately 21 days
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Rat DKK1 AccuSignal ELISA Kit - KOA0865
Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, DKK1, DKK-1, hDkk 1, hDkk1, mDkk1, SK
ELISA Kit
46.9 pg/ml - 3000 pg/ml

Target Details

DKK1  - View All DKK1 Products
Rat
Expression system for standard: NSO; Immunogen sequence: S23-H270
Natural and recombinant rat DKK1. There is no detectable cross-reactivity with other relevant proteins.
D3Z9J1 - UniProtKB
NP_001099820.1 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 419.pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml rat DKK1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each rat DKK1 standard solution and each sample is measured in duplicate.

Formulation

Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.