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Datasheet Protocol

Rat Cy3-SABC IHC Detection Kit

Rabbit Polyclonal

KOA0121
1 Kit
Rattus (Rat)
Rabbit
This product is discontinued

Background

Rat IgG Specific Cy3 Steptavidin-Biotin Complex Immunohistochemistry Detection Kit is a streptavidin-biotin immuno-enzymatic antigen detection system. This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, Cy3-conjugated streptavidin, and chromogen substrate. Rat Specific Cy3/DAB (ABC) kit includes reagents specifically designed for the use in the analysis of antigens in IHC and other immunodetection assays. The following protocol includes steps for high sesitivity analysis with low backgound using normal rabbit serum blocking reagent, biotinylated secondary antibody, and a Cy3-conjugated streptavadin.

Product Details

Rat Specific Cy3/SABC Detection Kit - KOA0121
Cy3 dye/SABC Kit Assay, StreptAvidin-Biotin Complex, Biotin Assay, Anti-Rat IgG antibody, Rabbit Anti-Rat IgG antibody, colormetric staining, immunohistochemistry staining
Rabbit
Cy3™
Polyclonal
Immunohistochemistry Kit

Target Details

Rattus (Rat)

Application Details

This kit contains concentrated products and will need dilution. See kit insert for details. Useful for Immunohistochemistry. IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.

Shipping & Handling

Wet Ice
Store at 4˚C for 12 months. Avoid freezing. See kit insert for complete instructions.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.