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Rat CCL11 - Eotaxin ELISA Kit

KOA0868
1 Kit
ELISA
Rat
15.6 pg/ml - 1000 pg/ml
$589.00 /Per Item
Out of stock
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Product Details

Rat CCL11/Eotaxin AccuSignal ELISA Kit - KOA0868
C-C motif chemokine 11, CCL 11, CCL11, CCL11_HUMAN, Chemokine (C-C motif) ligand 11, Chemokine C C motif ligand 11, Chemokine CC Motif Ligand 11, Chemokine ligand 11, Eosinophil Chemotactic P, Eosinophil chemotactic protein, Eotaxin, Eotaxin 1, SCYA 11, SCYA11
ELISA Kit
15.6 pg/ml - 1000 pg/ml

Target Details

CCL11  - View All CCL11 Products
Rat
Expression system for standard: E.coli; Immunogen sequence: H24-P97
Natural and recombinant rat Eotaxin. There is no detectable cross-reactivity with other relevant proteins.
P97545 - UniProtKB
NP_062078.1 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat Eotaxin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma(EDTA, citrate) to each empty well. It is recommended that each rat Eotaxin standard solution and each sample be measured in duplicate.

Formulation

Sodium EDTA

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Eotaxin, also known as CCL11, is a potent inducer of eosinophil chemotaxis and is considered as a selective ligand of the CC chemokine receptor 3 (CCR3), which is expressed on eosinophils, basophils, and Th2 lymphocytes. Eotaxin is assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. The gene maps to chromosome 17 and is expressed constitutively at high levels in small intestine and colon, and at lower levels in various other tissues. The deduced mature protein sequence is 66% identical to human monocyte chemoattractant protein-1, and 60% identical to guinea pig eotaxin.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.