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Mouse Flt-3 ligand ELISA Kit

KOA0173
1 Kit
ELISA
Mouse
31.2 pg/ml - 2000 pg/ml
$589.00 /Per Item
Availability: Ships in approximately 21 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Fms-related tyrosine kinase 3 ligand (FLT3LG), also known as Flt-3 ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3 ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor (SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3 ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3 ligand can control the development of Dendritic cells (DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3 ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

Product Details

Mouse Flt-3 ligand AccuSignal ELISA Kit - KOA0173
Flt 3 ligand, Flt 3L, Flt3 L, FLT3 LG, Flt3 ligand, Flt3L, FLT3L_HUMAN, Flt3lg, Fms related tyrosine kinase 3 ligand, Fms-related tyrosine kinase 3 ligand, SL cytokine
ELISA Kit
31.2 pg/ml - 2000 pg/ml

Target Details

FLT3LG  - View All FLT3LG Products
Mouse
Expression system for standard: NSO; Immunogen sequence: G27-R188
Natural and recombinant mouse Flt-3 ligand. There is no detectable cross-reactivity with other relevant proteins.

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse Flt-3 ligand standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse Flt-3 ligand standard solution and each sample be measured in duplicate.

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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