Human TFPI ELISA Kit
KOA0504
1 Kit
ELISA
Human
31.2 pg/ml - 2000 pg/ml
Shipping info:
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Product Details
Human TFPI AccuSignal ELISA Kit - KOA0504
EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor, TFPI, TFPI1_HUMAN, Tissue factor pathway inhibitor
ELISA Kit
31.2 pg/ml - 2000 pg/ml
Target Details
TFPI - View All TFPI Products
Human
Expression system for standard: NSO; Immunogen sequence: D29-K282
Natural and recombinant human TFPI. There is no detectable cross-reactivity with other relevant proteins.
P10646 - UniProtKB
NP_001027452.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human TFPI standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each human TFPI standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin(Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
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