Human MMP13 ELISA Kit
Human MMP13 Accusignal ELISA Kit
Datasheet is currently unavailable. Try again or CONTACT US
Datasheet

Human MMP13 ELISA Kit

KOA0275
1 Kit
ELISA
Human
156 pg/ml - 10000 pg/ml
Have a Question? Ask a Specialist
$552.00 /Per Item
Availability: Ships in approximately 21 days
Shipping info:

$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Application Note

Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human MMP13 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin) to each empty well. It is recommended that each human MMP13 standard solution and each sample be measured in duplicate.

Purity/Specificity

Natural and recombinant human total MMP13. There is no detectable cross-reactivity with other relevant proteins.

Human MMP13 AccuSignal ELISA Kit - KOA0275
CLG 3, CLG3, Collagenase 3, Collagenase3, MANDP1, Matrix metalloproteinase 13, Matrix metalloproteinase-13, MMP 13, MMP-13, Mmp13, MMP13_HUMAN, UMRCASE
MMP13
ELISA
Human
Expression system for standard: E.coli; Immunogen sequence:   L20-C471
ELISA Kit
156 pg/ml - 10000 pg/ml
<5 pg/ml
Heparin Sodium
1 Kit
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.

Invalid lot number

If you need help finding your CofA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.