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Human MIP-3 alpha - CCL20 ELISA Kit

KOA0261
1 Kit
ELISA
Human
15.6 pg/ml - 1000 pg/ml
$605.00 /Per Item
Availability: Ships in approximately 21 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Human MIP-3 alpha/CCL20 AccuSignal ELISA Kit - KOA0261
Beta chemokine exodus 1, Beta-chemokine exodus-1, C C motif chemokine 20, CC chemokine LARC, Ccl20, CCL20(2-70), CCL20_HUMAN, Chemokine (C C motif) ligand 20, CKb4, Exodus 1, LARC, Liver and activation regulated chemokine, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP 3 alpha, MIP 3a, MIP-3-alpha, MIP3 alpha, MIP3A, MIP3alpha, SCYA20, Small inducible cytokine A20, Small inducible cytokine subfamily A (Cys Cys) member 20, Small-inducible cytokine A20, ST38
ELISA Kit
15.6 pg/ml - 1000 pg/ml

Target Details

CCL20  - View All CCL20 Products
Human
Expression system for standard: E.coli; Immunogen sequence:  A27-M96
Natural and recombinant human MIP-3 alpha. There is no detectable cross-reactivity with other relevant proteins.
P78556 - UniProtKB
NP_001123518.1 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MIP-3 alpha standard solution and each sample be measured in duplicate.

Formulation

Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

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