Human Flt-3ligand ELISA Kit
KOA0172
1 Kit
ELISA
Human
15.6 pg/ml - 1000 pg/ml
Shipping info:
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Product Details
Human Flt-3ligand AccuSignal ELISA Kit - KOA0172
Flt 3L, Flt3 L, FLT3 LG, Flt3L, FLT3LG, Fms related tyrosine kinase 3 ligand, SL cytokine
ELISA Kit
15.6 pg/ml - 1000 pg/ml
Target Details
FLT3LG - View All FLT3LG Products
Human
Expression system for standard: sf21; Immunogen sequence: T27-P185
Natural and recombinant human Flt-3ligand. There is no detectable cross-reactivity with other relevant proteins.
P49771 - UniProtKB
NP_001191431.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.
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