Human Entactin - NID-1 ELISA Kit
KOA0517
1 Kit
ELISA
Human
62.5 pg/ml - 4000 pg/ml
Shipping info:
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Product Details
Human Nidogen-1/Entactin/NID-1 AccuSignal ELISA Kit - KOA0517
Entactin, NID-1, Nid1, NID1_HUMAN, Nidogen-1
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
NID1 - View All NID1 Products
Human
Expression system for standard: NSO; Immunogen sequence: L29-K1114
Natural and recombinant human Nidogen-1. There is no detectable cross-reactivity with other relevant proteins.
P14543 - UniProtKB
NP_002499.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human Nidogen-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human Nidogen-1 standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Nidogen-1(NID-1), also known as Entactin, is a protein that in humans is encoded by the NID1 gene. It is a member of the nidogen family of basement membrane glycoproteins. This gene is mapped to 1q42.3. Nidogen-1 is a component of the basement membrane alongside other components such as collagen type IV, proteoglycans(heparan sulfate and glycosaminoglycans), laminin and fibronectin. The protein interacts with several other components of basement membranes. Structurally it(along with perlecan) connects the networks formed by collagens and laminins to each other. It may also play a role in cell interactions with the extracellular matrix. Nidogen-1 also can serve as a bridge between the 2 most abundant molecules in the basement membrane: type IV collagen and laminin.
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