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Human CD23 - FCER2 ELISA Kit

KOA0549
1 Kit
ELISA
Human
31.2 pg/ml - 2000 pg/ml
$589.00 /Per Item
Availability: Ships in approximately 21 days
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Product Details

Human CD23/FCER2 AccuSignal ELISA Kit - KOA0549
BLAST 2, BLAST-2, C-type lectin domain family 4 member J, CD23, CD23 antigen, CD23A, CLEC4J, Fc fragment of IgE low affinity II receptor for (CD23), Fc-epsilon-RII, FCE2, Fcer2, FCER2_HUMAN, IGEBF, Immunoglobulin E-binding factor, Low affinity immunoglobulin epsilon Fc receptor, Low affinity immunoglobulin epsilon Fc receptor soluble form, Lymphocyte IgE receptor
ELISA Kit
31.2 pg/ml - 2000 pg/ml

Target Details

FCER2  - View All FCER2 Products
Human
Expression system for standard: NSO; Immunogen sequence: D48-S321
Natural and recombinant human CD23. There is no detectable cross-reactivity with other relevant proteins.
K3W4U1 - UniProtKB
NP_001193948.2 - NCBI Protein

Application Details

ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human CD23 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) or urine to each empty well. It is recommended that each human CD23 standard solution and each sample be measured in duplicate.

Formulation

Heparin Sodium

Shipping & Handling

Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.

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