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Datasheet Protocol

Hu FITC-HRP (F-ABC) IHC Detection Kit

Rabbit Polyclonal

1 Kit
H. sapiens (Human)
This product is discontinued


Human IgG Specific Fluorescein-Peroxidase Steptavidin-Biotin Complex Immunohistochemistry Detection Kit is a streptavidin-biotin immuno-enzymatic antigen detection system. This system involves the sequential incubation of the sample with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody that reacts with the primary antibody, fluorescein-peroxidase-conjugated streptavidin, and chromogen substrate. Human Specific FITC-HRP (F-ABC) IHC Detection Kit includes reagents specifically designed for the use in the analysis of antigens in IHC and other immunodetection assays. The provided protocol includes steps for high sesitivity analysis with low backgound using normal rabbit serum blocking reagent, biotinylated secondary antibody, and fluorescein-peroxidase-conjugated streptavadin.

Product Details

Human Specific FITC-HRP (F-ABC) Detection Kit - KOA0126
FITC + HRP Conjugated Kit Assay, StreptAvidin-Biotin Complex, peroxidase Conjugated, Fluorescein conjugated, Biotin Assay, Anti-Human IgG antibody, Rabbit Anti-Human IgG antibody, colormetric staining, immunohistochemistry staining
Immunohistochemistry Kit

Target Details

H. sapiens (Human)

Application Details

This kit contains concentrated products and will need dilution. See kit insert for details. Useful for Immunohistochemistry. IHC(P): 1:50-200, IHC(F): 1:50-200, ICC: 1:50-200. Optimal dilutions should be determined by end users.

Shipping & Handling

Wet Ice
Store at 4˚C for 12 months. Avoid freezing. See kit insert for complete instructions.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.