DyLight™ Multiplex 649/488/800 Trio Western Blot Kit

Background

The DyLight™ multiplex 649/488/800 Trio fluorescent Western blot kit is suited for simultaneous detection and quantification of specific protein populations in a biological sample. Using a combination of three antibodies selected for minimal cross reactivity, fluorescent detection method enables simultaneous quantitative analysis of multiple proteins within the same sample on the same blot. The DyLight™ multiplex 649/549/800 Trio Western blot kit contain all the necessary components that are optimized for the simultaneous detection of multiple proteins on the same blot using DyLight™-dye labeled secondary antibodies that are visualized in different fluorescence channels (649/549/800). The kit also includes blocking buffer, wash buffer, pre-stained protein standard and an incubation box for convenience and ultimate performance with minimal or no optimization. The fluorescent dyes such as DyLights™ when conjugated to secondary antibodies, offer a variety of benefits over traditional detection methods such as colorimetric and chemiluminescent detection. Multiplex detection using the correct lighting and filter conditions, enables the quantitation of multiple proteins and eliminates the need to strip and reprobe. Other benefits of fluorescent Western blotting include increased sensitivity, excellent signal stability over time as well as precise quantitative analysis with broader dynamic range and high linearity. Due to their exceptional photostability, DyLight™ dye conjugates can be archived and visualized several times without a decrease in signal.