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AccuSignal™ Nuclease ELISA Kit

Rabbit Polyclonal

1 Kit
Liquid and Lyophilized
0.03 to 20 ng/mL
$695.00 /Per Item
Availability: Call for availability
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35°C and 44°C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.

Product Details

AccuSignal™ Nuclease ELISA Kit - KJE-4001
Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA, Denarase
0.03 to 20 ng/mL

Target Details

Rockland’s AccuSignal™ Nuclease ELISA Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative detection of Nuclease/NucA in serum, plasma, and hybridoma cell supernatants. This kit contains: nuclease antibody coated 96-well strip plate, plate sealer, nuclease standard, biotinylated detection antibody, streptavidin-peroxidase conjugate, along with buffers and protocol. See protocol for details.

Application Details

Nuclease ELISA kit is designed for the quantitative detection of Nuclease/NucA in serum, plasma, and hybridoma cell supernatants. This kit was shown to positively detect commercially available nuclease variants, such as Benzonase, Denarase and other analogs.

Shipping & Handling

Wet Ice
The kit and reagents should be stored at 2-8°C. Allow reagents to reach room temperature (18-26°C) before use and may be used until the expiration date. It is recommended to aliquot the reconstituted standard solution and store it at -20°C to avoid freeze/thaw cycles.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.