WM115 Viable Cells (1 million cells)
WM115 viable cells
Human melanoma tumor cells
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WM115 Viable Cells (1 million cells)

WM115-01-0001 WM115-01-0005 WM115-01-0010
1 mL 5 x 1 mL 10 x 1 mL
Cellular Assay, WB
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$1,089.00 /Per Item
$3,629.00 /Per Item
$5,445.00 /Per Item
1 mL $1,089.00
5 x 1 mL $3,629.00
10 x 1 mL $5,445.00
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WM115 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line was established from a metastatic site (right anterior leg) in a 55-year-old female with superficial spreading melanoma. The subject displayed VGP with a Clark level III tumor with thickness of 2.24mm.This cell line features the specific V600D (Val600Asp) mutation at codon 600 in the BRAF gene. This cell line also expresses PTEN loss of function including hemizygous deletion. This cell line was derived from the same patient as the cell lines WM239A, WM165-1 and WM266-4. WM115 cell line originated from the primary tumor, and WM239A, WM165-1 and WM266-4 were from individual lymph-node metastases. WM115 cell line is wild type for N-RAS, c-KIT, and CDK4 genes. WM115 cells produce xenograft tumors when injected into immunocompromised mice.

Application Note

The key applications of these cell lines include genetic studies, xenograft production, drug testing, and drug target discovery. These cell line models can be used in various biological assays, and for identifying critical target genes, and cell signaling pathways.


Cells are sterile, validated by short tandem repeat profiling, and are tested as negative for mycoplasma. It is recommended that cell lines are tested for mycoplasma contamination and short tandem repeat (STR) profiling every 10 passages or each time a frozen seed stock is made. See cell culture protocol for additional details.

WM115 Viable Cells - WM115-01-0001
Melanoma, patient derived tumor, tumor models, skin cancer, xenograft, Human melanoma tumor cells
Cellular Assay, WB
Human Melanoma
Viable Cells
Hemizygous Deletion
Tumor Specialized Media with 2% HI-FBS
Cells should be maintained between 30 – 95% confluence in tumor specialized medium with 2% FBS; split cultures 1:3 every 7 days using 0.25% trypsin/EDTA. This cell line has a doubling time of 36-48 hours which may change slightly with the type of media used.
36°C with 5% CO2
Frozen Cell Suspension
1x10^6 cells/vial Count By Hemocytometer
Dry Ice
Cells are frozen with 90% FBS/10% DMSO solution at about 1x10^6 cells/ml. Store vial in liquid nitrogen upon arrival.
Expiration date is two (2) years from date of receipt.
Microfluidic model with air-walls reveals fibroblasts and keratinocytes modulate melanoma cell phenotype, migration, and metabolism. Lab Chip. ()
MX2 mediates establishment of interferon response profile, regulates XAF1, and can sensitize melanoma cells to targeted therapy. Cancer Med. ()
Cellular assay; WB, IB, PCA
Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis. Cancer Cell. ()
Melanoma Progression Inhibits Pluripotency and Differentiation of Melanoma-Derived iPSCs Produces Cells with Neural-like Mixed Dysplastic Phenotype. Stem Cell Reports. ()
IHC, ICC, Histology
Induction of paracrine signaling in metastatic melanoma cells by PPARγ agonist rosiglitazone activates stromal cells and enhances tumor growth. Cancer Res. ()

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