Bovine/Calf Buffy Coat Preparation
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Datasheet

Bovine/Calf Buffy Coat Preparation

R600-0100
100 µL
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$923.00 /Per Item
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Description

Background

Blood is a complex mixture of cells, proteins, metabolites and many other substances.  About 56% of human blood volume is comprised of cells, more than 99% of which are erythrocytes.  Human erythrocytes and thrombocytes (platelets, 0.5% blood components) do not contain nuclei.  Leukocytes (0.3% of blood components), also referred to as peripheral blood mononuclear leukocytes or PBMC, do contain nuclei.  Blood samples may vary widely in leukocyte (granulocytes, lymphocytes and monocytes) concentration depending on the health of the donor. Healthy blood contains less than 10E7 leukocytes per ml, while blood from an infected donor may have ten fold higher leukocyte concentrations. Buffy coat preparations are useful for researchers interested in immunology and infectious disease research.

Application Note

Buffy coat preparations are suitable for use as a source of nucleated PBMC. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). Buffy coat preparations are generally enriched for leukocytes (WBC) and contain trace amounts of red cells and other blood components. If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll™ gradient centrifugation. This product is prepared in a buffer composed of 20.5 mg/ml Dextrose, 4.2mg/ml Sodium Chloride, 8.0 mg/ml Citric Acid Tri-sodium, adjusted to pH 6.1.

Purity/Specificity

Buffy coat preparations are composed of leukocytes, trace amounts of red blood cells and other components. Buffy coat preparations are separated after a single low-speed centrifugation to remove the platelet rich plasma, buffy coat layer and erythrocytes (RBC). If desired, you may further purify this preparation of leukocytes by sedimentation using Percoll™ gradient centrifugation.

Storage Condition

Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.

BOVINE/CALF Buffy Coat Preparation - R600-0100
Bovine/Calf Buffy Coat Preparation, Bovine Buffy Coat Preparation, Calf Buffy Coat Preparation
Bovine
Non-sterile
Frozen Cell Pellet
Dry Ice
Expiration date is one (1) year from date of opening.
See application note.
None
None

Invalid lot number

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.