Quality Antibodies & Validation


Dr. Camilo Moncada: My name is Camilo Moncada. I'm the director of quality control here at Rockland Immunochemicals. So we test our our antibodies earlier when they are antiserum, we test them until the process of manufacturing. And finally, we test them as a product before they are released. We basically try to guarantee that we have specificity, sensitivity, and reproducibility in all of our products.

Dr. Karin Abarca Heidemann: Hi, I'm Karen Abarca. I'm the director of R&D and production here at Rockland. So our vision is to advance life sciences to make a better world. So for us, it's very, very important that our products and all our antibodies are validated properly depending on where they used for. So for us, it's very important that if they should work on Western blot that we validate them using Western blot assays. If they should work in ELISA, that they're properly validated in the assay like ELISA.

Dr. Camilo Moncada: What we do here is try to use complementary testing so that the information we can get from one particular immunoassay we can get it for another immunoassay. Let's take for example, Western blotting versus ELISA. In the Western blotting, we can easily detect the intended target under certain denaturing conditions, for example, but that's not the case in the ELISA as it would the antibody would not denature. So what we do is try to get that complementary information to let us know what's the performance of the antibody in different conditions.

Dr. Karin Abarca Heidemann: All our antibodies, all our products come with a CoA which will have in detail what we have done for testing on this antibody, for example, or even buffers. We have a technical team where all the direct, the questions, the technical directions would come them directly to that team. If that team cannot answer it because it's more a specialized question, usually it comes to one of our scientists and we have numerous scientists here at Rockland that can help guide technical questions.

Dr. Camilo Moncada: Overall the most valuable type of validation is using endogenous lysates and to use endogenous protein expression and silencing of the protein. We do that as much as possible. Again, we we use our results as like recombinant proteins or expressing cell lines.

Dr. Karin Abarca Heidemann: We have on our website numerous types of protocols. We have videos, we are starting to make videos for most of our applications to teach our researchers or our partners how to use our products.