ModDetect Antibody Panels

The first of its kind, Rockland’s ModDetect™ Anti-PS Monoclonal is a specialty antibody panel technology developed to detect phosphorothioate (PS) modifications independent of the sequence or location of the modification. This makes the Anti-PS panel useful for oligonucleotide therapeutic development, mRNA vaccine development, or research of genetic diseases or gene expression.

Advantages of the ModDetect™ Anti-PS Antibody

Rockland has a demonstrated history of successful generation of antibodies as analytical tools against different types of nucleic acid targets including modified backbones and nucleosides by way of fully customized, fit-for-purpose, anti-oligonucleotide antibodies. We have developed a panel of validated specialty reagents directed against phosphorothioate (with more to come) that can be used immediately, saving significant time compared to traditional programs that may take up to 12 months. And because these antibodies are fully designed, developed, optimized, and validated in-house, we can ensure supply chain security and respect of client confidentiality and intellectual property.

no sophisticated equipment

Uniquely Specific

Detect any PS modification independent of the sequence or location

no sophisticated equipment

Time Saving

Save time in your discovery and pre-clinical processes

no sophisticated equipment

Reproducible

In-house, US-based manufacturing ensures reproducible results

ModDetect™ Panels as Analytical Tools

ADME (Absorption, Distribution, Metabolism, Excretion)‚Äč
Discovery
Pre-Clinical / Non-Clinical
Types of Assays
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ELISA/ADA &
Immunogenicity Studies
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IP/Protein Binding
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In Vitro Cell Culture/
Potency Studies
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IHC/Biodistribution
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IF/Intracellular
Localization
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Figure 1. ELISA demonstrating the specificity of the immune response from one rabbit. Immulon HBX ELISA plates were coated with 100 ng/well of BSA conjugated 3’ or 5’ oligonucleotides with identical sequences. Anti-sera was diluted 1:100 then 3-fold serially diluted. Antibodies showed a preference to phosphorothioate backbone-modified oligonucleotides (Assay #2) over phosphodiester backbone (PO) oligonucleotides (Assay #1) independent of orientation.

Available Anti-Phosphorothioate (PS) (Mouse) Monoclonal Antibodies

Clone ID Isotype
PS01 IgG1 Kappa
PS02 IgG2a Kappa
PS03 IgG2a Kappa
PS04 IgG2a Kappa
PS05 IgG2a Kappa
PS06 IgG1 Kappa
PS07 IgG1 Kappa
PS08 IgG2a Kappa
PS09 IgG2a Kappa

Figure 2. ELISA Results of Anti-PS-Oligo Antibodies. Streptavidin-coated plates were coated in duplicate with 5 pmol/well of 5’Biotin-MOE-PS Gapmer. The starting dilution of antibody was 5 μg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. PS03 IC50: 11 ng/mL (Black circle), PS04 IC50: 9 ng/mL (red square), PS05 IC50: 10 ng/mL (Green triangle), PS09 IC50: 18 ng/mL (Blue triangle). Assay performed using Rabbit Anti-Mouse IgG HRP conjugated at 1:8,000 and TMB substrate.

 

Try our Early Access Program

The Early Access Program is available to grant immediate access to our ModDetect™ reagents as they are validated and released for use, potentially saving critical time compared with de novo development. If you are interested in participating, use the link below to request more information.

Note: Use licenses, both single and enterprise, are offered once these specialty reagents have been confirmed suitable for use for a specific drug substance and assay.

Request Early Access