Viral Vector Analytical Services
Viral Vector Analytical Services
Adeno-associated virus (AAV) is a leading platform for in vivo gene delivery, but successful AAV development requires robust, reproducible analytics. Whether you’re optimizing serotypes, preparing virus pools for assay development, or evaluating vector potency, Rockland provides flexible AAV services that meet the demands of modern gene therapy research. We specialize in upstream AAV production, research-grade purification, and analytical characterization with deep expertise in immunological and nucleic acid assay development.
Produce High-Yield AAV Virus Pools with Reliable Upstream Workflows
Efficient and reproducible AAV vector production starts with optimized upstream methods. Rockland supports your gene therapy development with flexible, research-grade AAV virus production services tailored to your serotype, payload, and expression system. We offer scalable upstream support for AAV production in HEK293 and other mammalian systems, including:
- Cell culture expansion in adherent or suspension formats
- Transient transfection with AAV packaging and helper plasmids
- Virus harvest from supernatants
- Small- to mid-scale AAV virus pool generation
Whether you're conducting serotype screening, expression optimization, or preparing for downstream purification, Rockland provides upstream AAV production support to accelerate your early-stage development.
Purify and Concentrate Your AAV Vectors for Research or Preclinical Use
Reliable purification and concentration are essential to produce clean, functional AAV vectors. Rockland offers a range of AAV purification services to support early-stage applications, assay development, and analytical testing. Our downstream capabilities include:
- Cesium chloride gradient ultracentrifugation for high-purity AAV separation
- Tangential flow filtration (TFF) for vector concentration and buffer exchange
- Clarification of harvested virus-containing supernatants
These methods yield clean, research-grade AAV samples suitable for QC testing, transduction assays, and preclinical validation. Whether you need to assess vector potency, perform neutralization testing, or develop a custom assay, our downstream AAV prep services ensure consistency and reproducibility.
Characterize Your AAV Vectors with High-Precision Analytics
Accurate AAV characterization is critical for evaluating vector quality, consistency, and potency. Rockland provides a suite of analytical services to quantify titer, assess infectivity, and verify purity for confident decision-making from R&D through preclinical studies. Our AAV analytical services include:
- Digital droplet PCR (ddPCR) for genome copy number quantification
- Capsid quantification via AAV ELISA
- Neutralization assays for evaluating vector inhibition by anti-AAV antibodies, supporting preexisting immunity testing or sample-based neutralization
- Infectivity and transduction assays using relevant target cells
- DNA purification and sterility testing (USP <71>)
Rockland delivers absolute quantification and improved reproducibility, ideal for comparing yields, validating batches, or developing IND-enabling workflows.