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Description
Background
DAB, a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and ability to give cleaner background as compared to amino ethylcarbazole (AEC). Specimens stained in DAB can be dehydrated, cleared, and mounted for permanent record keeping. Stable DAB/Plus is more sensitive and stable than traditional working DAB solutions. Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it, which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.
Application Note
Stable DAB/Plus offers several noteworthy improvements and benefits as compared with traditional working DAB solutions. Stable DAB/Plus is much more sensitive, providing the cost-effective option of diluting the primary antibody. Being stable for 5 days (as opposed to 6 hours for traditional DAB working solutions), Stable DAB/Plus allows the user the convenience of making one working solution for the entire work week. Hazardous waste generation from spent DAB solution is also significantly reduced. Stable DAB/Plus is ideal for high volume labs and automated stainers. Aliquot 20µL (1 drop) of DAB Chromogen Solution into 1mL of DAB/Plus Substrate Buffer in the Dropper bottle and mix thoroughly.
Purity/Specificity
Reagents provided: Concentrated Amber-Colored DAB Chromogen Solution 5mL, Clear Stable DAB/Plus Substrate Buffer 200mL, Empty Mixing Dropper Bottle.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.