1X Lysis Buffer
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Datasheet

1X Lysis Buffer

KBD-001
50 mL
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$77.00 /Per Item
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$50.00 to US & Canada for most products. Final costs are calculated at checkout.

Description

Background

Lysis Buffer is used to lyse cells under nondenaturing conditions. Cell Lysis Buffer is ideal to assist in Immunoprecipitation which allows for the purification by immunoprecipitation of recombinant proteins containing an epitope tag provided by the user. The user is able to further characterize the resultant purified protein by size, post-translational modification, western blot and other assays.

Application Note

Chill to 2 to 8 °C prior to use. Lysis Buffer is provided as a ready-to-use 1X solution containing 50 mM Tris Cl, 150 mM Sodium Chloride, 1 mM EDTA, 1% TRITON-X-100, pH 7.4. No preparation is required prior to use.

Purity/Specificity

Lysis Buffer was aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers.

1X Lysis Buffer - KBD-001
1X Lysis Buffer, 1X cell lysis buffer
Immunoprecipitation Kit
Liquid (sterile filtered)
1X
None
None
Wet Ice
See kit insert for complete instructions. Chill to 2 to 8 °C prior to use.
See kit insert for complete instructions.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.