Goat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed
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Datasheet

Goat IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Rabbit Polyclonal

605-4515
500 µg
Goat
Rabbit
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Description

Background

Anti-Goat IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH. F(ab')2 molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 molecules.

Application Note

This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:8,000 is suggested for this product.

Purity/Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum.  No reaction was observed against Goat IgG F(c) or Human Serum Proteins.

Anti-GOAT IgG F(ab')2 (RABBIT) Antibody Alkaline Phosphatase Conjugated (Min X Human Serum Proteins) - 605-4515
rabbit anti-goat IgG F(ab')2 ALP conjugated, rabbit anti-goat IgG F(ab')2 alkaline phosphatase conjugated, rabbit anti-goat IgG Fab2 ALP conjugated, rabbit anti-goat IgG Fab2 alkaline phosphatase conjugated
Goat
Rabbit
IgG F(ab')2
Alkaline Phosphatase (Calf Intestine)
Polyclonal
IgG
Goat IgG F(ab')2 fragment
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Wet Ice
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.