F(ab')2 Dog IgG (H&L) Antibody
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F(ab')2 Dog IgG (H&L) Antibody

Rabbit Polyclonal

1 mg
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$159.00 /Per Item
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F(ab')2 Anti-Dog IgG (H&L) Antibody generated in rabbit detects specifically Dog IgG. This secondary Anti-Dog IgG antibody is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis. Anti-Dog IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.

Application Note

F(ab')2 Anti-Dog IgG (H&L) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.


F(ab')2 Anti-Dog IgG (H&L) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum.  No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).

F(ab')2 Anti-DOG IgG [H&L] (RABBIT) Antibody - 704-401-002
Rabbit F(ab')2 Anti-Dog Antibody, Rabbit Fab2 Anti-Dog Antibody, Rabbit Fab'2 Anti-Dog Antibody
IgG (H&L)
IgG F(ab')2
Dog IgG whole molecule
Liquid (sterile filtered)
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
Wet Ice
Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.