Datasheet

WWP2 protein-GST fusion

Name: WWP2 protein-GST fusion
Brand: Rockland Immunochemicals
Availability: OutOfStock

Item No. 009-001-U60

Size: 50 µg

Reactivity: H. sapiens (Human)

Origin: Human

Expressed in: Sf9 cells

This product is discontinued

Background

WWP2 or WW domain containing E3 ubiquitin protein ligase 2 is a member of the Nedd4 family of E3 ligases, which play an important role in protein ubiquitination. WWP2 contains four WW domains and may play a role in multiple processes including chondrogenesis and the regulation of oncogenic signaling pathways via interactions with Smad proteins and the tumor suppressor PTEN. WWP2 is an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination (1). WWP2 which is also called AIP2 can bind to atrophin-1 (2). WWP2 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cardiovascular Disease, and Cell Cycle research.

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Specifications for WWP2 protein-GST fusion

Product Details

Description: WWP2 recombinant protein-GST fusion protein - 009-001-U60

Synonyms: AIP2, WWp2-like, NEDD4-like E3 ubiquitin-protein ligase WWP2, Atrophin-1-interacting protein 2, AIP2, WW domain-containing protein 2

Species of Origin: Human

Expressed in: Sf9 cells

Type: Recombinant Protein

Target Details

Gene Name: NEDD4

Reactivity: H. sapiens (Human)

Purity/Specificity: Recombinant full-length human WWP2 was expressed by baculovirus in Sf9 insect cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.

Database Links:

NCBI - NM_007014

Application Details

Application Note: WWP2 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. WWP2 Protein is suitable for use in Western Blot. Expect a band approximately ~130kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 0.1 µg/µl

Buffer: See application note.

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiration: Expiration date is one (1) year from date of receipt.

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.