Datasheet is currently unavailable. Try again or CONTACT US
Datasheet Protocol

Ultrapure Collagen I for plate coating - Human Placenta

009-G70-MF0
8 mL
Liquid (sterile filtered)
Cellular Assay
Human
$1,225.00 /Per Item
Availability: Ships next business day
Shipping info:

$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.

Background

Human Collagen Type I is one of a family of proteins found particularly in the flesh and connective tissues of mammals (approximately one-third of the body's total protein). Over two dozen types of collagen have been described; Type I is the most abundant form in the body. Collagen Type I is found in scar tissue, tendons, the skin, arterial walls, the corneas, muscles, cartilage, and in the organic parts of bones and teeth. Human Collagen Type I is ideal for investigators involved in extracellular matrix proteins and osteoporosis.

Product Details

Ultrapure Collagen I for plate coating - Human Placenta - 009-G70-MF0
Type I collagen, collagen 1, Human collagen, Alpha 1 type I collagen, Alpha 2 type I collagen, COL1A1, COL1A2, Collagen alpha-1(I) chain, Collagen alpha-2 (I) chain, plate coating solution
Human

Target Details

COL1A1
Ultrapure Human Collagen Type I Solution is purified from human placenta and shown to be 99% pure.

Application Details

Cellular Assay  - View References
Ultrapure Human Collagen Type I solution has bee tested in cell culture and is suitable for plate coating. Enough to coat 10 (ten) T75 plates.

Formulation

1.0 mg/mL by dry weight
0.013M HCl
None
None

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is six (6) months from date of receipt.

What is the source of the collagen proteins?

Collagen proteins are purified from various sources including bovine or human placenta, skin, nasal tissue, and knee cartilage. The purity of the protein is analyzed by SDS-PAGE.

How are the collagen proteins purified?

The proteins were purified by a proprietary method that includes mechanical separation and salt precipitation. Due to the nature and close association of certain collagen proteins, it is difficult to fully separate each collagen.

In what format are the collagen proteins provided?

Collagen proteins are provided in liquid format at a concentration of around 1 mg/mL. The collagen proteins are solubilized, maintaining their native structure. No enzymatic treatment is performed. The low pH of the buffer is essential in maintaining the solubility of the collagen protein.

In what buffer are the collagen proteins provided?

0.5 M Acetic Acid, pH 2.5 with 0.01% (w/v) Sodium Azide.

What are the recommended SDS-PAGE conditions for collagen proteins?

The recommended buffer for SDS-PAGE is 2X SDS buffer with 8 M Urea. The low pH of the buffer maintains the solubility of the collagen proteins. Also, low-density gels such as 3-8% Tris Acetate should be used. Collagen proteins can be reduced with DTT.

What are the recommended storage conditions for collagen proteins?

Collagen proteins should always be stored at 4°C. It is important to never let the collagen proteins freeze because this will degrade the protein. Please be sure to maintain 4°C temperature during assays such as SDS-PAGE.

How was the concentration determined?

The concentration of the collagen proteins was measured by absorbance via nanodrop at 205 nm. When measuring concentration, please be sure to blank with the same buffer as the protein product (0.5 M Acetic Acid).

Are collagen proteins suitable for cell culture?

Since no testing for endotoxin or other contaminants was performed, we do not recommend the use of this product for cell culture.

Invalid lot number

If you need help finding your CoA, contact us

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: Ultrapure Collagen I for plate coating - Human Placenta