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UBE2J1 protein-GST fusion

009-001-U38S 009-001-U38
20 µg 50 µg
Sf9 cells
$325.00 /Per Item
$665.00 /Per Item
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.


UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. UBE2J1 play an important role in the homeostatic regulation of MHC class I assembly and expression (1). HRD1 and UBE2J1 associate in a complex together with non-β2m bound MHC class I heavy chains, Derlin 1, and p97 and discriminate misfolded MHC class I from conformational MHC I-β2m-peptide heterotrimers. UBE2J1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

Product Details

UBE2J1 recombinant protein-GST fusion protein - 009-001-U38
CGI-76, HSPC153, HSPC205, HSU93243, NCUBE-1, NCUBE1, UBC6, Ubc6p, Ubiquitin-conjugating enzyme E2 J1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E
Sf9 cells
Recombinant Protein

Target Details

Recombinant full-length human UBE2J1 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.

Application Details

UBE2J1 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. UBE2J1 Protein is suitable for use in Western Blot. Expect a band approximately ~70kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.


Liquid (sterile filtered)
0.1 µg/µL
See application note.

Shipping & Handling

Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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