UBE2H protein-HIS Epitope
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Datasheet

UBE2H protein-HIS Epitope

009-001-U36S 009-001-U36
20 µg 50 µg
Human
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$165.00 /Per Item
$256.00 /Per Item
20 µg $165.00
50 µg $256.00
Availability: Ships in approximately 30 days Availability: Ships in approximately 30 days
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Description

Background

UBE2H or ubiquitin-conjugating enzyme E2H is a member of the E2 ubiquitin-conjugating enzyme family which is located on chromosome 7 (1). UBE2H is known to act on histones and cytoskeletal proteins, both involved in the degenerative pathway of the motor neuron. UBE2H expression is increased during erythroid differentiation of hCD34 (+) cells. Tal1 transcription factor, which is essential for the development of the hematopoietic system and plays a role during definitive erythropoiesis in the adult, activates UBE2H expression, whereas Tal1 knock-down reduced UBE2H expression and ubiquitin transfer activity (2). UBE2H Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cancer, Cell Cycle, and Neurobiology research.

Application Note

UBE2H Protein is stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. UBE2H Protein is suitable for use in Western Blot. Expect a band approximately ~21kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Purity/Specificity

Recombinant full length human UBE2H was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

UBE2H recombinant protein-HIS Epitope - 009-001-U36
E2-20K, UBC8, UBCH, UBCH2, Ubiquitin-conjugating enzyme E2 H, Ubiquitin-protein ligase H
UBE2H
Human
Protein
Recombinant Protein
Liquid (sterile filtered)
0.1 µg/µL
See application note.
Dry Ice
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Expiration date is one (1) year from date of opening.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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